Mass Isolation of Germinal Vesicles from Starfish Oocytes*. (germinal vesicle/nucleus/oocyte/starfish/mass isolation)
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چکیده
منابع مشابه
Isolation and derivation of mouse embryonic germinal cells.
The ability of embryonic germinal cells (EG) to differentiate into primordial germinal cells (PGCs) and later into gametes during early developmental stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum (dpc) mouse embryos. Developing gonadal ridges from...
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The starfish oocyte is relatively clear optically, and its nucleus, termed the germinal vesicle, is large. These characteristics allowed studies by confocal microscopy of germinal vesicle breakdown during maturation in living oocytes. Three fluorescent probes for cytoplasmic components were used: fluorescein 70 kDa dextran, which does not cross the nuclear pore of immature oocytes and probably ...
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Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...
متن کاملChromatin modifications in the germinal vesicle (GV) of mammalian oocytes.
The nucleus of eukaryotic cells is organized into functionally specialized compartments that are essential for the control of gene expression, chromosome architecture and cellular differentiation. The mouse oocyte nucleus or germinal vesicle (GV) exhibits a unique chromatin configuration that is subject to dynamic modifications during oogenesis. This process of 'epigenetic maturation' is critic...
متن کاملZoology: W. S. Vincent the Isolation and Chemical Properties of the Nu- Cleoli of Starfish Oocytes
The function of several of the discrete protoplasmic structures has been more completely understood where the structure itself has been isolated and studied by direct biochemical methods.' Progress toward the understanding of the chemical nature and of the function of the nucleolus has been hindered by the lack of a suitable technique for the isolation of nucleoli in quantities suitable for dir...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1985
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1985.00277.x